Fig. 7: Cooperation between STAT5 and BATF promotes antitumor immunity.
From: STAT5 promotes accessibility and is required for BATF-mediated plasticity at the Il9 locus
a Naive CD4+ T cells were isolated from OTII (CD45.2) mice and differentiated into Th17 cells for 5 days. Retrovirus expressing caSTAT5 (tagged with hNGFR) and/or BATF (tagged with Thy1.1) was transduced on day 1. Transduced cells were sorted by gating on reporter-positive and CD4-positive cells before transfer to B16-OVA-bearing Boy/J (CD45.1) mice. b Tumor development was assessed by tumor size from day 8–17. c, d Tumor weight at day 18 and visual examination. e Flow cytometric analysis of tumor infiltrating immune cells. P-values are comparison for CD8 T cells. Data are the mean ± SEM. n = 5 for control group, n = 4 for caSTAT5 and caSTAT5 + BATF group. One-way ANOVA with a post hoc Tukey’s test was used to generate p-values for b and c. Two-way ANOVA with Sidak’s multiple comparison test was used to generate p-values for e.
