Fig. 4: Optoribogenetic control of the mammalian cell cycle.
a shRNA variants used to control cyclin B1 gene expression. Blue: aptamer domain; orange: siRNA domain. b Percentages of HEK293PAL cells in G2/M phase of the cell cycle when transfected with indicated shRNAs targeting cyclin B1. c shRNA variants used to control CDK1 gene expression. Blue: aptamer domain; green: siRNA domain. d Percentages of HEK293PAL cells in G2/M phase of the cell cycle when transfected with indicated shRNAs targeting CDK1. b N = 20 (SHCB1, SHCB1m) or 6 (SHCB2, SHCB3). d N = 10. b, d Each biologically independent experiment was performed in duplicates. b The identity of SHCB1 and SHCB1m was blinded and double-blinded in one experiment, each. d The identity of SHCDK1 and SHCDK1m was blinded and double-blinded in one experiment, each. b, d Wilcoxon two-sided signed-rank test was used for statistical analysis. e, Representative western blot image showing cyclin B1, CDK1 and GAPDH protein expression after transfection with the indicated shRNAs (for complete blots see Supplementary Fig. 21). e, N = 4. e One biologically independent experiment was performed in duplicates, all others once. f, g Quantification of cyclin B1 and CDK1 protein levels using pixel densitometry. f, g N = 3. f, g All biologically independent experiments were performed once. f, g Cohen’s d effect size was used for statistical analysis. Values were normalized to non-transfected cells incubated in darkness (Untransfected). b, d, f, g Gray bars: cells incubated under light conditions, black bars: cells incubated under dark conditions. Values are means ± s.d. Source data for (b, d, f, g) are provided as source data file.
