Fig. 6: Eicosapentaenoic acid (EPA) and linoleic acid (LA) supplementation activates p38-MAPK. | Nature Communications

Fig. 6: Eicosapentaenoic acid (EPA) and linoleic acid (LA) supplementation activates p38-MAPK.

From: Polyunsaturated fatty acids and p38-MAPK link metabolic reprogramming to cytoprotective gene expression during dietary restriction

Fig. 6

a GC-MS analysis revealed that PUFAs are differentially regulated when drl-1 is knocked down in WT. The levels of these PUFAs change in a similar manner when drl-1 is knocked down in sek-1(km4). n = 7 biologically independent samples. Data are presented as mean values +/− std. dev. Unpaired two-tailed t test. b, c The Pcyp-35B1::gfp worms were grown on control or sek-1 RNAi plates supplemented with EPA (b) or LA (c) since hatching. PUFA supplementation increased the expression of Pcyp-35B1::gfp when worms are grown on control RNAi but not on sek-1 RNAi. Multiple overlapping images were acquired at 100X magnification to cover the entire worm body and stitched together to generate a contiguous image. One of two (LA) or three (EPA) independent experiments shown. d Western blot analysis of Pcyp-35B1::gfp grown on control or sek-1 RNAi showing that EPA and LA supplementation upregulates phospho-PMK-1 levels, in a sek-1-dependent manner. One of three independent experiment shown. Source data are provided as a Source data file. All experiments were performed at 20 °C.

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