Fig. 2: Expression of piUL49 in HSV-1-infected cells. | Nature Communications

Fig. 2: Expression of piUL49 in HSV-1-infected cells.

From: Identification of a herpes simplex virus 1 gene encoding neurovirulence factor by chemical proteomics

Fig. 2

a Schematic diagram of the genome of wild-type HSV-1 (line 1) and the locations of the UL49 (VP22) and iUL49 (piUL49) CDSs (line 1). The domain(s) of iUL49 detected by MS-analyses (line 3) or used to generate antibodies to piUL49 (line 4). The nucleotide and amino-acid sequences of VP22 (line 5) and piUL49 (line 6) are indicated. b, c Vero cells mock-infected or infected with Venus-iUL49 (b), Venus-iUL49ΔM (b), MEF-iUL49 (c), or MEF-iUL49ΔM (c) for 12 h at an MOI of 10 were analyzed by immunoblotting with antibodies to GFP (b), VP23 (b), Flag (c), gB (c), or β actin (b, c). d Vero cells mock-infected or infected with wild-type HSV-1(F), iUL49ΔM, iUL49ΔM-rep, VP22ΔM, or VP22ΔM-rep for 12 h at an MOI of 10 were analyzed by immunoblotting with antibodies to piUL49, VP22, ICP8, gB, or β actin. e, f Vero cells mock-infected or infected with wild-type HSV-1(F) (e, f), HSV-1(17) (e), HSV-1(KOS) (e) or HSV-1(VR3) (e), ΔVP22/ΔiUL49 (f), or ΔVP22/ΔiUL49-rep (f) for 12 h at an MOI of 10 were analyzed by immunoblotting with antibodies to piUL49, VP22, or β actin. Digital images are representative of three independent experiments (b–f).

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