Fig. 5: piUL49 is required to compensates for low cellular dUTPase activity. | Nature Communications

Fig. 5: piUL49 is required to compensates for low cellular dUTPase activity.

From: Identification of a herpes simplex virus 1 gene encoding neurovirulence factor by chemical proteomics

Fig. 5

a, b Viral titers of sh-Luc-HEp-2 or sh-hDUT-HEp-2 were infected with wild-type HSV-1(F), iUL49ΔM, iUL49ΔM-rep or iUL49ΔM/F-hDUTn for 36 h at an MOI of 10 (a) or for 60 h at an MOI of 0.01 (b), respectively, and assayed. Each value is the mean ± SEM of four independent experiments. The indicated P-values were obtained using one-way ANOVA followed by Tukey’s test. n.s. not significant. c, d Viral titers in the brains (c) (n = 16) or LD50 values (d) of 3-week-old female ICR mice intracranially infected with iUL49ΔM-rep, iUL49ΔM, or iUL49ΔM/F-hDUTn were determined as described in Fig. 3i–l. Each value is the mean ± SEM for each group. The indicated P-values were obtained using one-way ANOVA followed by Tukey’s test. n.s. not significant. Dashed line indicates the limit of detection. e dUTPase activities (dpm/μg of total protein) in eyes, TGs and brains of nine 4-week-old female ICR mice. Each value is the mean ± SEM of four independent experiments. The indicated P-values were obtained using one-way ANOVA followed by Tukey’s test. n.s. not significant. f–h Four-week-old female ICR mice were infected ocularly with 3 × 106 PFU/eye of iUL49ΔM or iUL49ΔM-rep. Viral titers in the eyes (f), TGs (g), or brains (h) of infected mice at 1 day (n = 8), 2 day (n = 11), 3 day (n = 11), 5 day (n = 12), and 7 day (iUL49ΔM-rep, n = 16; iUL49ΔM, n = 12) post-infection were assayed. Dashed line indicates the limit of detection. n.d. not detected. The indicated P-values were obtained using an unpaired two-tailed Student’s t test (eyes and TGs at 1 day, TGs at 2 day, and eyes and brains at 5 day) and Welch’s t-test (eyes and brains at 2 day, eyes, TGs and brains at 3 and 7 day, and TGs at 5 day). n.s. not significant. i Four-week-old female ICR mice were infected ocularly with 3 ×  106 PFU/eye of iUL49ΔM-rep (n = 14) or iUL49ΔM (n = 15) and monitored daily for survival for 21 days. The indicated P-values were obtained using Log-rank test. Source data are provided as a Source Data file.

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