Fig. 3: Tet1 and Tet2 bind to specific mRNAs within the transcriptome.

a Diagram illustrating CRISPR-mediated tagging of endogenous Tet1, Tet2, and Tet2ΔRBD proteins (CRISPR KI) and RIP-Seq experiment design. b Many mRNAs are bound by Tet1. Scatterplot showing the log-fold enrichment over input after Tet1-bound RNA immunoprecipitation. c RNA-binding targets of Tet2. Scatterplot showing the log-fold enrichment over input after Tet2-bound RNA immunoprecipitation. d Tet2 RBD is involved in mRNA targeting. Scatterplot showing the log-fold enrichment over input after Tet2ΔRBD-bound RNA immunoprecipitation. e Tet2 binding to many RNAs depends on Tet2 RBD. Venn diagram showing the overlap between Tet2- and Tet2ΔRBD-bound RNA targets identified by RIP-Seq. f Many common Tet1 and Tet2 targets. Venn diagram showing the overlap between Tet1- and Tet2-bound RNA targets identified by RIP-Seq. g 5hmC targets are often bound by both Tet1 and Tet2. Stacked bar chart showing the overlap between 5hmC-containing transcripts and RNA targets bound by both Tet1 and Tet2, only Tet1, or only Tet2. All RIP-Seq were performed at least in biological duplicate.