Fig. 1: Cryo-EM single-particle reconstruction of the mature Zika virus.

a Representative micrographs from 2128 total micrographs in the dataset of vitrified MR-766 ZIKV purified from Vero-E6 cells. Micrographs contained particles with large regions of immature, uncleaved prM (blue arrow), mosaic immature (red arrows), and mostly mature particles (green arrows). b Surface rendered density map of the icosahedrally averaged reconstruction with two asymmetric units (white solid and dashed lines) and symmetry axes (red font) labeled to highlight the raft subunit comprised of three EM dimer pairs (top). The slabbed map (bottom) is radially colored according to the key (r = 110–230 Å) to emphasize the layering of the mature virion, including glycoprotein (blue–green), lipid bilayer (yellow), and capsid protein, and genome RNA (red). c, top: A central section illustrates the quality of the density in the 4.0 Å 60-fold symmetric reconstruction. c, bottom: The zoom quarter map section shows the conserved transmembrane E–M helices within the lipid bilayer (yellow).