Fig. 5: Complex layer-wise dysregulation of genes involved in glutamate-mediated excitation in the cortex of epileptic patients. | Nature Communications

Fig. 5: Complex layer-wise dysregulation of genes involved in glutamate-mediated excitation in the cortex of epileptic patients.

From: Identification of epilepsy-associated neuronal subtypes and gene expression underlying epileptogenesis

Fig. 5: Complex layer-wise dysregulation of genes involved in glutamate-mediated excitation in the cortex of epileptic patients.The alternative text for this image may have been generated using AI.

a Notable layer-wise upregulation or downregulation of genes involved in glutamate-mediated excitation. b Overview of smFISH that shows CKAMP44 (SHISA9) and RORB mRNAs in the temporal cortex of control (nonepileptic) and epileptic brains. The RORB probe was used to label layer L4, thereby allowing the identification of the cortical layer structure (together with DAPI staining). Note the higher number of CKAMP44 mRNA molecules across all layers in the epileptic brain. c, d Representative image and quantification of CKAMP44 mRNA molecules in control (nonepileptic) and epileptic temporal cortices demonstrate upregulation of CKAMP44 in RORB-positive neurons belonging to L4_Rorb and L5–6_Fezf2_Lrrk1 principal neurons (one-tailed Mann–Whitney test, 98 cells in two sections of two control brains and 215 cells in three sections of three epileptic brains).

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