Fig. 8: Neuronal subtypes most affected by epilepsy based on integrative analysis.

a Six metrics—expression similarity, cell-type composition, number of changed GO terms, enrichment in GWAS genes, enrichment in epilepsy genes, and number of DE genes—are aggregated into a single score. The six metrics are shown on the y axis, ordered by the metric weight, where the weight is represented by the blue vertical colorbar on the right and by the color transparency of the rows. Cell types grouped into interneurons/principal neurons are shown on the x axis, ordered by the total score (green horizontal colorbar on the top). The colors on the heatmap represent the strength of the effect: 0—not affected, 1—affected, 2—highly affected, 3—most affected. For a rational of how we assigned the level of effect and weight for each metric, see “Methods”. b Clustering of cell subtypes based on enrichment of certain GO terms (similar to Fig. 3d). Enlarged inlet shows co-clustering of cell subtypes with the most affected transcriptomes (based on the metric in Fig. 8a).