Fig. 3: LRRK2 G2019S alters Ca²⁺-storage and Ca²⁺ response to IFN-γ.

ER Ca²⁺ release was measured after SERCA inhibition with thapsigargin (TPH, 500 nM) in human iPSC-derived neurons (G2019S and isogenic controls) using fura-2 AM ratiometric Ca²⁺ imaging. a Individual Ca²⁺ signaling traces of representative isogenic LRRK2 G2019S and control neurons are shown. The change in fluorescence was normalized to the corresponding baseline fluorescence prior to TPH stimulation. b Quantification of Ca²⁺ peak amplitude (Δ-Ca²⁺ release) is shown (mean ± SEM, two-tailed t-test, ****P < 0.0001; n = 56 control and 59 G2019S individual cells examined over three independent experiments; non-normalized data are provided in the Data Source File). c Individual Ca²⁺ signaling traces of representative isogenic LRRK2 G2019S and control neurons pretreated with 200 IU/mL IFN-γ for 24 h or left untreated. d Quantification of TPH-mediated Δ-Ca²⁺ release is shown (mean ± SEM, two-way ANOVA, Bonferroni post hoc, ****P < 0.0001; n = 56 control, 42 IFN-γ treated control, 59 G2019S and 59 IFN-γ treated G2019S individual cells examined over three independent experiments).