Fig. 4: Superresolution imaging of LRRK2-dependent microtubule network. | Nature Communications

Fig. 4: Superresolution imaging of LRRK2-dependent microtubule network.

From: Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells

Fig. 4

a Representative images of iPSC-derived neurons carrying the LRRK2 G2019S mutation and corresponding isogenic controls (3 independent experiments were performed and 5-7 cells were analyzed per condition, per experiment). Upper panel: total internal reflection fluorescence (TIRF) images of cell soma microtubules stained for β-III tubulin and probed with Alexa Fluor 647-conjugated secondary antibody; middle panel: superresolution dSTORM images of microtubule networks; lower panel: extraction of filaments network, composite filament network obtained from automated extraction by SIFNE. Scale bars, 5 μm. b–h Quantitative analysis of the microtubule network. b cell soma area (mean ± SEM, two-tailed t-test, n = 33 control and 36 G2019S individual cells examined over three independent experiments); c number of filaments per cell (mean ± SEM, two-tailed t-test, n = 33 control and 36 G2019S individual cells examined over three independent experiments); d mean filament length (mean ± SEM, two-tailed t-test, **P = 0.0023, n = 31 control and 35 G2019S individual cells examined over three independent experiments); e number of junctions (mean ± SEM, two-tailed t-test, n = 33 control and 35 G2019S individual cells examined over three independent experiments); f normalized distributions of junction density as a function of distance from cell edge (mean ± SEM, multiple t-test, *P = 0.0005, n = 33 control and 36 G2019S individual cells examined over three independent experiments); g normalized filament curvature distribution (mean ± SEM, multiple t-test, *P = 0.0003, n = 33 control and 36 G2019S individual cells examined over three independent experiments); h distribution of mean curvature as a function of distance from cell soma edges Kolmogorov–Smirnov test, **P = 0.0015; n = 33 control and 36 G2019S individual cells examined over three independent experiments); on the right, a more detailed representation of the values in close proximity to the cell edge (0–0.5 μm).

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