Fig. 1: Cell-based assays and structural analysis of PF846-stalled termination complexes.
From: Selective inhibition of human translation termination by a drug-like compound
a Luciferase reporter assays of CDH1–NPN* (black circles) and CDH1–GCV* (magenta circles) stable cell lines in response to PF846 with different concentrations of 0, 1, 3, 5, 7, 10, 20, 50, and 100 μM (data present mean ± s.d., n = 3 independent experiments). The vertical line indicates the PF846 concentration when the fitted line crosses 0.5. From the CDH1–GCV* data, the IC50 of nonspecific inhibition is at least 100 μM PF846. b Structure of CDH1–NPN* RNC in the rotated state bearing A/P-site (dark green) and P/E-site tRNAs (slate blue), with mRNA (magenta), the 40S subunit (light cyan) and 60S subunit (gray). A close-up view of the mRNA and tRNAs is shown to the right. c Cryo-EM reconstruction of CDH1–NPN* RNC in the nonrotated state, with eRF1 (slate blue), and P/P-site tRNA (orange). A close-up view of the mRNA, tRNA, and eRF1 is shown to the right.
