Fig. 2: Overview of PF846-stalled nascent chains in the ribosome exit tunnel.
From: Selective inhibition of human translation termination by a drug-like compound
a The PF846-stalled nascent chain from the rotated RNC (light blue). Surface representations of the segmented cryo-EM density for PF846 and ribosomal proteins are indicated. b Cryo-EM density for the stalled nascent chain within the nonrotated RNC (purple). Surface representations of the segmented cryo-EM density for eRF1, P-site tRNA, PF846, and ribosomal proteins are indicated. c Western blots of CDH1–GCV* and CDH1–NPN* RNCs in the presence (+) or absence (−) of 50 μM PF846 on different time scales at room temperature. Puromycin treatment was performed on pelleted RNCs at room temperature for 1 h with a final concentration of 1 mM puromycin. The experiment shown in e was repeated three times independently with similar results.
