Fig. 5: Flux measurements in synchronized cell populations reveal decreased ATP synthesis and ATP levels in mitosis relative to G2. | Nature Communications

Fig. 5: Flux measurements in synchronized cell populations reveal decreased ATP synthesis and ATP levels in mitosis relative to G2.

From: Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division

Fig. 5: Flux measurements in synchronized cell populations reveal decreased ATP synthesis and ATP levels in mitosis relative to G2.The alternative text for this image may have been generated using AI.

a Schematic of cell cycle synchronization procedure. Oxygen-consumption rates were measured using cells arrested in mitosis due to the time needed to obtain measurements. Lactate efflux and ATP and ADP levels were measured in cells progressing through mitosis. All results were normalized by the number of cells in mitosis and G2. b Oligomycin-sensitive oxygen-consumption rate, i.e., mitochondrial ATP synthesis rate, in G2 and mitotic cells (N = 7–8 independent synchronizations and cultures). c Lactate efflux rate in G2 and mitotic cells (N = 4 independent synchronizations and cultures). d ATP levels in G2 and mitotic cells (N = 5 independent synchronizations and cultures). e ADP levels in G2 and mitotic cells (N = 5 independent synchronizations and cultures). For panels (b–e), data depict mean ± SD (compound errors after accounting the mean values for imperfect cell cycle synchrony). Unpaired, two-tailed Student’s t test was used for statistical analysis.

Back to article page