Fig. 2: TMPRSS2 is part of a T2 inflammation-induced mucus secretory network.

a WGCNA identified networks of co-regulated genes related to mucus secretory function (black), T2 inflammation-induced mucus secretory function (pink), and canonical T2 inflammation biomarkers (saddle brown). TMPRSS2 was within the pink network. Select pathway and cell-type enrichments for network genes are shown. Enrichment p-values were obtained from a one-sided hypergeometric test. Benjamini–Hochberg correction was used to control for false discovery rate. b Scatterplot revealing a strong positive correlation between TMPRSS2 expression and summary (eigengene) expression of the T2 inflammatory, mucus secretory network. p-values were obtained from the two-sided Pearson correlation test. c Scatterplot revealing a strong positive correlation between TMPRSS2 expression and summary (eigengene) expression of the canonical T2 inflammation biomarker network. p-values were obtained from the two-sided Pearson correlation test. d Box plots revealing strong upregulation of TMPRSS2 expression among T2-high (n = 364) compared to T2-low (n = 331) subjects. Differential expression testing between T2-high and T2-low groups was performed using a two-sided Wilcoxon test. e Scatterplot revealing a strong negative correlation between ACE2 expression and summary (eigengene) expression of the T2 inflammation mucus secretory network. p-values were obtained from the two-sided Pearson correlation test. f Scatterplot revealing a strong negative correlation between ACE2 expression and summary (eigengene) expression of the canonical T2 inflammation biomarker network. p-values were obtained from the two-sided Pearson correlation test. g Box plots revealing strong downregulation of ACE2 expression among T2-high (n = 364) compared to T2-low (n = 331) subjects. Differential expression testing between T2-high and T2-low groups was performed using a two-sided Wilcoxon test. Box centers give the median, upper and lower box bounds correspond to first and third quartiles and the upper/lower whiskers extend from the upper/lower bounds up to/down from the largest/smallest value, no further than 1.5 × IQR from the upper/lower bound (where IQR is the inter-quartile range). Data beyond the end of whiskers are plotted individually.