Fig. 1: SnRNA-seq analysis from adult slow and fast skeletal muscles.
From: Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers
a Graphical scheme of the experiments used for snRNA-seq analysis of adult skeletal muscles. b Uniform Manifold Approximation and Projection (Umap) diagram from snRNA-seq from adult skeletal muscle. MuSC skeletal muscle stem cells, FAPs fibro-adipogenic progenitors, MTJ myotendinous junction, NMJ neuromuscular junction. c Umap diagram of snRNA-seq data with myonuclei only. Myonuclei are separated into five clusters: slow, fast, MTJ, NMJ, and unidentified myonuclei. The slow and fast myonuclei are called body nuclei and are surrounded by dotted lines. Five subcategories of fast myonuclei are clustered. d Heatmap of genes upregulated (yellow) and downregulated (violet) in the myonuclei expressing the different slow and fast Myh isoforms. The values correspond to z-scores of normalized counts.
