Fig. 4: Serine supplementation alleviates atg2Δ-dependent tlag on ethanol media.
a Wild-type (black) and atg2Δ (blue) cells were grown on glucose media (upper left) or ethanol media with indicated supplements. b Growth of WT (black) and atg2Δ (blue) cells on ethanol media with (broken lines) or without (solid lines) supplemented serine. c Dose–response curve of serine concentration vs atg2Δ-dependent tlag. Mean values (red points) of three independent determinations (grey points) is shown, with logistic fitting to mean values. d Recovery of autophagy-dependent tlag in response to the provision of ethanol media with indicated supplements. Representative individual growth curves are shown in a and b. Boxplots are shown as median (middle bar), 25th and 75th percentiles (upper and lower limits of the box) and 1.5 × interquartile range (whiskers), with sample sizes indicated on the plot. Indicated p-values were calculated using the two-sided Student’s t-test with Welch modification.
