Fig. 5: Mitochondrial respiration is perturbed in the absence of bulk autophagy. | Nature Communications

Fig. 5: Mitochondrial respiration is perturbed in the absence of bulk autophagy.

From: Autophagy facilitates adaptation of budding yeast to respiratory growth by recycling serine for one-carbon metabolism

Fig. 5

a Oxygen consumption rates of WT (black) and atg2Δ (blue) cells at various points of respiratory growth. The growth of strains is shown in the top panel, whereas substrate (ethanol-dependent) oxygen consumption and decoupled (+FCCP) oxygen consumption rates are shown in the lower panels. Cells grown on serine-supplemented media are indicated by dashed lines. b The proportion of of WT and atg2Δ cells characterised by high mitochondrial membrane potential (ΔΨm), with strains and conditions as indicated in a. Membrane potential was determined by flow cytometry of DiOC6 stained cells, as described in the methods. The gating strategy is shown in Supplementary Fig. 12. All data are single determinations (n = 1).

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