Fig. 6: Autophagy-dependent tlag is caused by delayed mitochondrial translation initiation.
a Overview of one-carbon metabolism and the mechanism of protein translation initiation in mitochondria. b Boxplots of indicated strain tlag on ethanol media in the presence or absence of serine. Boxplots are shown as median (middle bar), 25th and 75th percentiles (upper and lower limits of the box) and 1.5 × interquartile range (whiskers), with sample sizes indicated on the plot. c Formylation of mitochondrial initiator Met-tRNA for WT and atg2Δ cells grown in the absence or presence of supplemented serine. Samples were collected at the time points indicated in the upper panel and subjected to 32P northern blotting of whole-cell RNA extracts. The formylated species (fMet-tRNAfMet) is indicated. 18 S RNA is provided as a loading control d 35S labelling of mitochondrial translation products in WT and atg2Δ cells grown on ethanol media in the absence or presence of serine. Samples were collected at the time points indicated in the upper panel before labelling was conducted in the presence of the cytosolic translation inhibitor cycloheximide. Coomassie staining is provided as a loading control. Representative data from two independent experiments is shown.
