Fig. 5: In vivo biosynthesis of fluorosugars in engineered P. putida. | Nature Communications

Fig. 5: In vivo biosynthesis of fluorosugars in engineered P. putida.

From: A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

Fig. 5: In vivo biosynthesis of fluorosugars in engineered P. putida.The alternative text for this image may have been generated using AI.

a Growth curves of P. putida KT2440::FRS-T7RNAP containing the plasmids modules described in Fig. 4b, compared to the growth profile of P. putida KT2440::FRS-T7RNAP bearing an empty vector (pSEVA231). F− addition to the cultures is indicated with a black arrow. Green arrows indicate the points when cultures were harvested to test 5′-FDRP biosynthesis. Average and standard deviations of the specific growth rates (μ) after the addition of F− are shown in the plot for each engineered strain. Error bars correspond to standard deviations of three different biological replicates. b Extracellular concentration of 5′-FDRP after 2, 20, 24, and 48 h of induction of the synthetic circuit with NaF. Error bars correspond to standard deviations of three different biological replicates. CDW cell dry weight. Data are presented as mean values and error bars correspond to standard deviations from three biological replicates. Source data are provided as a Source Data file.

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