Fig. 6: Antibodies to PIX-1 detect PIX-1a on a western blot and localize to muscle cell boundaries, M-lines, and dense bodies. | Nature Communications

Fig. 6: Antibodies to PIX-1 detect PIX-1a on a western blot and localize to muscle cell boundaries, M-lines, and dense bodies.

From: The Rho-GEF PIX-1 directs assembly or stability of lateral attachment structures between muscle cells

Fig. 6

a Schematic representation of domains in the predicted isoforms PIX-1a and PIX-1b and regions used as immunogens to generate antibodies. b Western blot detection of a protein of expected size for PIX-1a from wild type and from pix-1(gk893650) [P190S] but not from deletion or nonsense pix-1 mutants. Anti-paramyosin was used as a gel loading control. An image of the entire blot reacted with anti-paramyosin is available in the Source Data Files. c Antibodies to PIX-1 localize to muscle cell boundaries and with less intensity to M-lines and dense bodies. Each strain was co-stained with antibodies to PAT-6 and PIX-1, and imaged by confocal. Note the lack of PIX-1 immunostaining in the nonsense and deletion pix-1 mutants, but strong and disorganized staining in pix-1(gk893650) [P190S]. Arrowheads point to a muscle cell boundary. Each image is a representative image obtained from at least 2 fixation and immunostaining experiments, and imaging at least three different animals of each strain. Scale bar, 10 μm.

Back to article page