Fig. 5: Elevated brown adipose tissue thermogenic capacity and white adipose tissue browning in Y524S mice.
From: Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation
a Gross anatomy for the interscapular brown adipose tissue (iBAT) from WT and YS littermates. b, c Tissue to body weight ratio of iBAT resected from littermate pairs of WT and YS in male (n = 42 pairs, b) and female (n = 36 pairs, c) mice. d Representative PET-CT scan of WT and YS littermate. e Quantification of total iBAT activity in WT and YS littermates (n = 16 pairs). f Representative PET-CT scans from the interscapular region of WT and YS mice from various age groups. g–i Age related decline of body weight-adjusted total iBAT activity in WT (n = 28, g) and YS (n = 17, h) mice, and comparison between WT and YS within age groups (i). j Relative UCP1 protein levels in iBAT from WT (n = 10) and YS (n = 10) littermates. k H&E stain images of subcutaneous inguinal white adipose tissue (iWAT) from WT and YS littermate. Morphological changes are consistent across three independent littermate pairs. l Relative protein levels of UCP1 in iWAT from WT (n = 22) and YS (n = 22) littermates, using iBAT as a positive control. P values are indicated as analyzed by paired t test (b, c, e), F-test for deviation from zero-rate constant of nonlinear regression (g, h), and two-way ANOVA with Sidak’s multiple comparisons test (i), and Welch’s t test (j, l). All statistical tests are two-sided. R2 values are indicated to quantify goodness-of-fit to nonlinear regression with variable slope (g, h). Data are represented as mean ± standard deviation (i, j, l), or asymmetrical 95% confidence intervals (CI) from nonlinear best-fit curve (g, h). Mice were within the controlled age range (male: 12.0 ± 0.3-week-old (b), and female: 11.9 ± 0.3 week old (c)) at the time of study, except for the age-dependent experiments (g–i). Display ranges of standard uptake value (SUV) for PET and Hounsfield unit (HU) for CT are as indicated (f). Scale bars are 5 mm from the gross anatomy images (a), 10 mm from the PET-CT scans (d, f), and 100 μm from the histological images (k). Source data are provided as a source data file.
