Fig. 2: Photostability of mFAPs compared to AcGFP1.
From: Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
a, b Normalized fluorescence intensity images for four regions of interest (ROIs) acquired under continuous wave imaging at ~0.885 Hz (1.13 s frame−1) shown for frame 1 (left) and frame 200 (right) of fixed COS-7 cells expressing endoplasmic reticulum-targeted a mFAP2a labeled with 50.0 µM DFHBI compared to b AcGFP1. Scale bars represent 10 microns. c, d Means (lines) and s.d. of the means (shading) of the normalized summed pixel intensities of four ROIs (n = 4) under continuous wave imaging (~0.885 Hz) for c mFAP2a labeled with 50.0 µM DFHBI (~330-fold above Kd) (blue), 500 nM DFHBI (~3.3-fold above Kd) (orange), 50.0 µM DFHBI-1T (~8.6-fold above Kd) (green), or 500 nM DFHBI-1T (~12-fold below Kd) (red) compared to AcGFP1 (black), and d mFAP2b labeled at 50.0 µM DFHBI (~28-fold above Kd) (blue) or 500 nM DFHBI (~3.6-fold below Kd) (orange) compared to AcGFP1 (black). Source data is available for Fig. 2.
