Table 2 Fluorescence response to acetylcholine stimulation of HEK293 cells expressing cytosolic Ca2+-responsive mFAPs or GCaMP6f.

From: Incorporation of sensing modalities into de novo designed fluorescence-activating proteins

Sensor

Peak \(| {\frac{{{\Delta} {\boldsymbol{F}}}}{{{\boldsymbol{F}}_0}}} |\)

[DFHBI] (µM)

Regions of interest

Fluorescence response to

 increased [Ca2+]

EF2n_mFAP2a

0.12 ± 0.092

20.0

15

Negative

EF4n_mFAP2b

0.42 ±  0.14

43.3

10

Negative

EF4n_mFAP2a

0.46 ±  0.12

43.3

15

Negative

GCaMP6f

11 ± 1.8

0.00

12

Positive

  1. The mean and s.d. of the mean of peak absolute values of the fluorescence fold-change (peak \(| {\frac{{{\Delta} F}}{{F_0}}} |\)) upon acetylcholine (ACh) stimulation over the indicated number of regions of interest surrounding single cells (3 technical replicates per sensor; Fig. 5e and Supplementary Fig. 18).
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