Fig. 2: Homozygous S85C knock-in mice reach humane endpoint at over one year of age.
From: Selective neuronal degeneration in MATR3 S85C knock-in mouse model of early-stage ALS
a Homozygous S85C animals (both males and females) exhibited significant differences in weight starting at around 15 and 21 weeks of age for males and females, respectively. Each dot represents mean ± SEM, where males: n = at least 10 Matr3+/+, 10 Matr3S85C/+, 5 Matr3S85C/S85C; females: n = at least 8 Matr3+/+, 10 Matr3S85C/+, 8 Matr3S85C/S85C (exact n numbers for each age are presented in Supplementary Information). Two-way ANOVA, Dunnett correction for multiple comparisons, see Supplementary Information for p-values at each age (*p < 0.05). b The phenotype score for wild-type, heterozygous and homozygous S85C male and female animals at over 1 year of age. Mice with a score of 60 were considered to be at the endpoint. Each dot represents a single animal (males: n = 10 Matr3+/+, 12 Matr3S85C/+, 6 Matr3S85C/S85C; females: n = 10 Matr3+/+, 12 Matr3S85C/+, 11 Matr3S85C/S85C), with data presented as mean ± SEM. Significance was determined by unpaired two-tailed t-test; ****p < 0.0001. c Size difference between wild-type and homozygous S85C mice at endpoint. Source data are provided as a Source Data file.
