Fig. 2: T cell immunity is generated in the vast majority of patients.

a, d The mean (n = 18) pre-immunization (Pre) and highest post-vaccination (Post) frequency of antigen-specific IFN-γ⁺ (a) or IL-17⁺ T (d) cell frequencies (per million PBMCs plated) that recognize the vaccine epitopes as well as the whole FRα protein. Post-vaccination samples included up to week 19. P values were calculated using the 2-sided test Wilcoxon matched pairs at a significance level of P ≤ 0.05. b, e The % of patients that responded to vaccine epitopes with IFN-γ⁺ or IL-17⁺ T cell responses, respectively. c, f The distribution of IFN-γ⁺ and IL-17⁺ T cell responses to the individual epitopes, respectively. g Correlation analysis heatmap comparing the magnitude of maximal peptide-specific IFN-γ⁺ T cells to each other and to the maximal FRα protein-specific IFN-γ⁺ T cell response. Inset values are Spearman’s Rho. h The same for IL-17⁺T cell responses. All correlations ≥0.45 were P < 0.05 (Benjamini-Hochberg adjusted two-sided test, exact P values are indicated in Supplementary Tables 5 and 6). i Correlation plot between the protein-specific IFN-γ⁺ T cell response and the IL-17 analyses between the highest FRα protein-specific IFN-γ⁺ and highest FRα protein-specific IL-17⁺ T cells. j Correlation analysis between the number of peptides to which IFN-γ⁺ T cells were generated and the number of peptides to which IL-17⁺ T cells were generated. k Correlation plots between the vaccine IFN-γ score (The sum of the individual patient T cell response to the epitopes) and tumor FRα expression. Inset values for (i–k) are Spearman’s Rho coefficient (r) and P value. l Correlation plots between the vaccine Th17 score (The sum of the individual patient T cell response to the epitopes) and tumor FRα expression. Inset values are Pearson’s Rho coefficient (r) and P value. Each symbol in (i–l) represents a unique patient (n = 18 for each panel). In some cases the symbols overlap obscuring each other. Inset best-fit lines were calculated with non-linear least squares regression and intended for data trend visualization.