Fig. 4: Genomic origin firing pattern changes are sequence specific.

DNA sequences under each of the origin replication peak that match the ARS consensus sequence (ACS) motif were obtained from OriDB^13,19. Genome-wide statistical analysis was performed to check the dependence of origin firing peak height on dinucleotide identity at positions 29–30, which were chosen based on the results of our MPOS analysis (Fig. 2c), using a one-way ANOVA test for dependence. There were 5 dinucleotide variants that occurred 3 or less times in 9 annoated ACSs were removed prior to this analysis. In the remaining n = 211 annotated ACSs, there were 5 dinucleotide variants at 29–30; these were analyzed and the peak heights for each dinucleotide are plotted. Asterisks denotation: *p < 0.05, **p < 0.01, ***p < 0.001. a Genome-wide statistical analysis results for all ten strains. b–e Box plots for Orc4 wild-type (b) and three of the orc4 mutant strains (c–e). Y-axis shows genomic log₁₀ peak heights. Each dot denotes an annotated ACS. Box plots elements: the minimum height, first (lower) quartile, median, third (upper) quartile, and maximum height. Diamonds denotes outliers that exhibited aberrantly large values. The “AG” dinucleotide at position 29–30 is not utilized in mutants that change Y486 to alanine or glutamine.