Fig. 2: TROLL-2 and TROLL-3 are markers of breast cancer progression.

a, b Quantification of the ISH scores of TROLL-2 (a) and TROLL-3 (b) in the indicated TMA. Data were analysed with two-way ANOVA. Asterisk vs. normal breast tissue (NB), P < 0.005. Section sign vs. lobular hyperplasia (LH), P < 0.005. Hash vs. ductal carcinoma in situ (DCIS), P < 0.005. IDC = invasive ductal carcinoma. c, d Kaplan–Meier curves of overall breast cancer survival data based on the levels of TROLL-2 (c) and TROLL-3 (d) in tumours of the indicated TMA. e Representative H&E stained cross sections of mammary adenocarcinomas derived from MDA MB-231 cells infected with the indicated shRNAs. f Tumour volume quantification of the tumours described in e. n = 10 tumours per group, Asterisk vs. shNT (non-targeting), P < 0.005, two-tailed Student’s t test. g Representative H&E stained cross sections of lung colonies derived from CA1D cells infected with the indicated shRNAs. h Quantification of the lung colonies described in g. n = 5 mice for all groups, asterisk vs. NT, P < 0.005, two-tailed Student’s t test. All boxplots represent the individual data points, median and whiskers (min to max method). Source data are provided as Source data file.