Fig. 9: Protein ubiquitination is reduced post MI in CHIPS20E mice. | Nature Communications

Fig. 9: Protein ubiquitination is reduced post MI in CHIPS20E mice.

From: CHIP phosphorylation by protein kinase G enhances protein quality control and attenuates cardiac ischemic injury

Fig. 9: Protein ubiquitination is reduced post MI in CHIPS20E mice.The alternative text for this image may have been generated using AI.

a Example protein ubiquitination gel and total protein (TP) loading gel from CHIPS20E and littermate controls (WT) after Sham or MI intervention. Summary results: n = 6 biological replicates, 2WANOVA; interaction term p = 4 × 10−7, Tukey mct: *p ≤ 3 × 10−9, †3 × 10−4. b Example immunoblot for total CHIP protein expression at baseline and post-MI in the peri-infarct zone from WT and CHIPS20E mice. Summary: 6 biological replicates, 2WANOVA, Tukey mct: *p ≤ 5 × 10−10. c CHIP gene expression (Stub1/Gapdh) same study measured in Sham and post MI mice. 2WANOVA results provided in figure. d Filter trap assay for insoluble proteins probed for ubiquitin which are increased after MI in WT but maintained at low levels in CHIPS20E myocardium. N = 6 biological replicates, 2WANOVA, Tukey mct: *p = 12 × 10−7, †p = 1.9 × 10−6. Source data are provided as a Source Data file. Individual data and mean ± SD shown in each summary panel.

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