Fig. 3: eAf1521 strongly enriches for Ser-ADP-ribosylation.
From: Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins
a Box plot representing MS1 intensities of all ADPr-modified peptides of untreated and H2O2-treated HeLa cell lysate enriched with either WT Af1521 or eAf2151. Box extends from the 25th to 75th percentiles, the band indicates the median and whiskers extend to min and max (n = 3 biochemical independent samples). The statistical analysis and p value calculation was performed with two-tailed Student’s t test (**p < 0.01, **p < 0.001). b Scatter plots depicting MS1 ADPr-modified peptide intensities of selected S-ADPr-modified peptides. The modification confidence of the amino acid acceptor sites are >95%. Data are presented as mean (n = 3 biochemical independent samples). The statistical analysis and p value calculation was performed with two-tailed Student’s t test (*p < 0.05, **p < 0.01, ns not significant). c Scatter plots depicting MS1 ADPr-modified peptide intensities of selected R-ADPr-modified peptides identified in all untreated and H2O2-treated HeLa lysates. The modification confidence of the amino acid acceptor sites are >95%. Data are presented as mean (n = 3 biochemical independent samples). The statistical analysis and p value calculation was performed with two-tailed Student’s t test (ns not significant). Source data are provided as a Source data file.
