Fig. 2: mFlt1 is not required for plgf-induced arterial diameter growth. | Nature Communications

Fig. 2: mFlt1 is not required for plgf-induced arterial diameter growth.

From: The GEF Trio controls endothelial cell size and arterial remodeling downstream of Vegf signaling in both zebrafish and cell models

Fig. 2

a–c In vivo confocal imaging of aISV in WT (a); mft1−/− mutant, mfltka605, (b); mft1−/− combined with plgf gain of function plgfmusc (c); n = 58,64 and 57 aISVs per genotype derived from three autonomous experiments. The areas indicated by the red dotted boxes are displayed at higher magnification in the lower panels. d Quantification of images in a–c. Mean ± s.e.m, unpaired two-sided students t-test, n = 7,13, and 24 aISVs for indicated scenario. **p = 0.0051, ***p < 0.001. Note: significantly increased aISV diameter in mflt1−/−+plgfmusc scenario. e–g In vivo confocal imaging of aISV in plgfmusc (e), plgfmusc concomitant with morpholino knock-down of flt1 (f; plgfmusc + flt1-MO), flt1−/− mutant, flt1ka601 (g). The areas indicated by the red dotted boxes are displayed at higher magnification in the lower panels. h Quantification of images in e–g. mean ± s.e.m, unpaired two-sided students t-test, n = 40,19, and 13 aISVs for indicated scenario. ns not significant, **p < 0.01. Scale bar; 25 µm in all panels. hpf hours post fertilization, aISV intersegmental artery, MO morpholino.

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