Fig. 9: Trio and endoglin interact to determine 3dpf aortic EC size.

a Dorsal aorta diameter at 3dpf in endoglin morphants (magenta bar), endoglin morphants injected with 0.8 ng Trio targeting morpholino (blue bar), endoglin morphants treated with Trio inhibitor ITX3 (dark green bar) and WT treated with ITX3 (light green bar). Note that loss of Trio or inhibiting Trio reduced diameter growth in endoglin morphants. Mean ± s.e.m, unpaired two-sided students t-test, n = 8, 9, 8, 10 independent embryos per group. **p = 0.0011, ***p = 0.001. b Aorta EC surface area at 3dpf in endoglin morphants (magenta bar), endoglin morphants injected with 0.8 ng Trio targeting morpholino (blue bar), endoglin morphants treated with Trio inhibitor ITX3 (dark green bar) and WT treated with ITX3 (light green bar). Note that loss of Trio or inhibiting Trio reduced EC surface area in endoglin morphants. Mean ± s.e.m, unpaired two-sided students t-test, n = 18, 20, 22, 16 cells derived from six independent embryos per group; ***p < 0.001; **p = 0.0055; *p = 0.0100.