Fig. 2: Positional cloning of susa2-1.
From: SUSA2 is an F-box protein required for autoimmunity mediated by paired NLRs SOC3-CHS1 and SOC3-TN2
a Schematic diagram of chromosome 5 indicating the region where susa2-1 was mapped to. The numbers of recombinants obtained during mapping are indicated in parentheses. b List of potential susa2 candidate non-synonymous mutations in the mapped region obtained from next-generation whole-genome sequencing analysis. c The gene structure of SUSA2. Boxes and lines represent exons and introns, respectively. Gray boxes represent untranslated regions, and black ones represent coding sequences. The asterisk indicates the G-to-A mutation in susa2-1, and the arrows indicate the sites of T-DNA insertions in susa2-2 and susa2-3. d Morphology of 3.5-week-old Col-0, saul1-1, susa2-1 saul1-1, susa2-2 saul1-1, susa2-3 saul1-1, and susa2-1, susa2-2, susa2-3 plants. Plants were grown on 1/2 MS medium for 10 days and then transplanted on soil for two weeks before the picture was taken. e The predicted protein domains of SUSA2. The N-terminal F-box domain and C-terminal ACTIN domain are indicated with black boxes. The asterisk indicates the position of the conserved G252E amino acid (a.a.) change caused by the susa2-1 mutation. f Morphology of 3.5-week-old Col-0, saul1-1, susa2-1 saul1-1, and two independent transgenic plants with SUSA2::SUSA2 transformed into susa2-1 saul1-1. Plants were grown on 1/2 MS medium for 10 days and then transplanted on soil for two weeks before the picture was taken. g SUSA2 expression in the indicated plants in f as determined by RT-PCR and normalized to ACTIN7 (ACT7). Error bars represent means ± SD (one-way ANOVA, SPSS Statistics, n = 3, p < 0.01). Experiments were repeated three times with similar results.
