Fig. 5: Both susa2-2 and hsp90.3-1 suppress the temperature-dependent autoimmunity of chs1-2. | Nature Communications

Fig. 5: Both susa2-2 and hsp90.3-1 suppress the temperature-dependent autoimmunity of chs1-2.

From: SUSA2 is an F-box protein required for autoimmunity mediated by paired NLRs SOC3-CHS1 and SOC3-TN2

Fig. 5: Both susa2-2 and hsp90.3-1 suppress the temperature-dependent autoimmunity of chs1-2.The alternative text for this image may have been generated using AI.

a Morphology of 4-week-old Col-0, chs1-2, susa2-2 chs1-2, and susa2-2. Plants were grown on soil for 3 weeks and then moved to 18 °C for 1 week to induce the autoimmunity of chs1-226. b, c RT-PCR analysis of the expression of PR1 (b) and PR2 (c) in the indicated genotypes. ACT7 was used to normalize the transcript levels. Error bars represent means ± SD (one-way ANOVA, SPSS Statistics, n = 3, p < 0.01). Experiments were repeated three times with similar results. d Ion leakage measurement of the plants as shown in a. Ion leakage was calculated as the percentage of the conductivity before autoclaving over that after autoclaving. Eight leaf discs were used per genotype in 22.5 ml H2O. Error bars represent means ± SD (one-way ANOVA, SPSS Statistics, n = 3, p < 0.01). Experiments were repeated three times with similar results. e Morphology of 4-week-old Col-0, chs1-2, hsp90.3-1 chs1-2, and hsp90.3-1 plants. Plants were grown on soil for three weeks and then moved to 18 °C for 1 week before the picture was taken.

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