Fig. 6: Direct conversion of HPBECs to hiHepPCs. | Nature Communications

Fig. 6: Direct conversion of HPBECs to hiHepPCs.

From: Direct reprogramming of human umbilical vein- and peripheral blood-derived endothelial cells into hepatic progenitor cells

Fig. 6

a Schematic diagram of the experimental procedure. b Immunofluorescence staining of ALB was conducted for mock-infected HPBECs at day 45 and HPBEC-derived hiHepPCs at day 45 and passage (P) 10 after retrovirus infection. Representative morphologies and fluorescence images of these two types of cells are shown. White broken lines surround hiHepPC colonies. Scale bars, 100 µm. The graph shows the percentages of ALB+ cells observed in individual cultures of HPBECs at day 45 and hiHepPCs at day 45, P3, and P10 after retrovirus infection. The data obtained from two biologically independent experiments using different blood samples are shown in the graph. Data represent the mean ± SD (n = 3 independent assays). c Co-immunofluorescence staining of ALB with E-CAD, AAT, ASGPR1, or CYP3A4 and of AFP with HNF4A; PAS staining; and oil red O staining were conducted for HPBECs and HPBEC-derived hiHepPCs. Representative morphologies of these two types of cells are also shown. White broken line surrounds a hiHepPC colony. In addition, HPBEC-derived hiHepPCs incorporated and excreted ICG. DNA was stained with DAPI. Scale bars, 50 µm. Source data are provided as a Source Data file.

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