Fig. 3: Metformin promotes CD8+ T-cell SRC, mitochondrial mass, survival, and FAO.
From: Metformin enhances anti-mycobacterial responses by educating CD8+ T-cell immunometabolic circuits
a Oxygen consumption rate (OCR) and spare respiratory capacity (SRC) in spleen CD8+ T cells from control and metformin-treated WT mice in response to different drugs. n = 4 control mice, n = 5 metformin-treated mice. b OCR in spleen CD8+ T cells from control and metformin-treated Cxcr3−/− mice. n = 6 mice/group. c Mitotracker green staining of CD8+ T cells from control and metformin-treated WT mice. MFI - median fluorescence intensity. n = 4 mice/group. d TMRM staining of CD8+ T cells from control and metformin-treated WT mice. n = 4 mice/group. e Experimental schematic of adoptive transfer of CD8+ T cells into congenic WT mice. f Frequency of CD45.1+ cells in the spleen and lymph node of recipient mice. n = 4 mice/group. g OCR in spleen CD8+ T cells from control and metformin-treated WT mice in response to different drugs under low glucose (LG) condition in the presence and absence of palmitate. n = 5 mice/group. h, i OCR in spleen CD8+ T cells from metformin-treated WT mice in response to different drugs under LG condition in the presence of palmitate. h n = 5 mice/group; i n = 8 mice/group. j Glucose uptake (2-NBDG) in CD8+ TM cells, analyzed by flow cytometry. n = 5 mice/group. Data in a, c, d, f, g, and j are presented as mean ± SD, two-tailed Mann–Whitney U test.
