Fig. 3: Binding properties of anti-ZIKV NS1 mAbs. | Nature Communications

Fig. 3: Binding properties of anti-ZIKV NS1 mAbs.

From: Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy

Fig. 3

Binding to NS1 by anti-NS1 mAbs. Binding to cell surface-associated NS1 was assessed by flow cytometry following staining at 4 °C of live, ZIKV-infected Vero cells with serial dilutions of murine (a–c) or human (e, f) mAbs. a Representative flow cytometry plots showing binding of anti-NS1 mAbs to NS1 on the cell surface. A minimum of 5000 cells was collected per sample. The percentage of cells staining (b, e) or the mean fluorescence intensity (MFI) (c, f) is shown as the average of two replicates and is representative of two or three experiments; error bars represent the mean ± SD. d, g Binding of mAbs to recombinant, soluble NS1 was assessed by ELISA. Absorbance values for each concentration of mAb are the average of duplicates and representative of two experiments. EC50 values for binding are reported in Supplementary Table S2. Source data are provided as a Source Data file for Fig. 3b–g.

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