Fig. 6: Cx40+ lineage tracing identifies that a maximal level of Nkx2-5 is required during embryonic stages for proper Purkinje network development. | Nature Communications

Fig. 6: Cx40+ lineage tracing identifies that a maximal level of Nkx2-5 is required during embryonic stages for proper Purkinje network development.

From: Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network

Fig. 6

a and b Genetic tracing of Cx40+ cells labeled at E18.5 by Tam injection. Confocal images of whole-mount CNTN2-immunostaining of Cx40-CreERT2::R26R-YFP opened-left ventricle at P21. a″ and b″, Immunostaining for YFP and CNTN2 on control, or Nkx2-5+/− mice shows E18.5 Cx40+-derived cells that participate to the PF network. Scale bars = 1 mm; a’ and b’ are high magnifications of left bundle branch; a″ and b″ are high magnifications of PF. c–e Strategy to induce conditional deletion of one copy of Nkx2-5 allele (Nkx2-5flox/+) using Cx40-CreERT2 line as inducible Cre driver. Deletion is performed at E18.5 when Cx40+ cells are mostly PF cells or E10.5 before the massive recruitment of late progenitors. R26R-YFP reporter line is used to compare the genetic tracing of Cx40+-derived cells within P21 control, Nkx2-5+/− and Nkx2-5flox/+ hearts (N = 7 of each genotype for E18.5 and N = 3 for E10.5). e Confocal imaging of left PF network. Scale bars = 500 µm; arrowheads indicate non-conductive Cx40+-derived cells.

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