Table 1 Data collection and structure statistics for SAXS analysis.
Data collection parameters | Sb23 | RBD | Sb23+RBD |
|---|---|---|---|
Data collection parameters | |||
Instrument | EMBL P12 (PETRA III, DESY, Hamburg) | ||
Beam geometry (mm2) | 0.2 × 0.12 | ||
Wavelength (nm) | 0.124 | ||
s range (nm−1) | 0.03–5.0 | ||
Exposure time (s) | 4 (20 × 0.2 s) | ||
Temperature (K) | 293 | ||
Concentration range (mg ml−1) | 0.37–4.0 | ||
Structural parameters | |||
Rg (nm) (from P(r)) | 2.2 ± 0.1 | 3.2 ± 0.2 | 3.7 ± 0.2 |
Rg (nm) (from Guinier plot) | 2.1 ± 0.1 | 3.1 ± 0.2 | 3.5 ± 0.2 |
Dmax (nm) | 8.0 ± 0.5 | 13 ± 1 | 15 ± 1 |
Porod volume estimate, Vp (nm3) | 20 ± 2 | 66 ± 2 | 86 ± 5 |
Molecular weight determination (kDa) | |||
From Porod volume (Vp/~1.6) | 13 ± 1 | 37 ± 5 | 54 ± 3 |
From consensus Bayesian assessment | 15 ± 3 | 41 ± 1 | 53 ± 6 |
From I(0) | 21 ± 2 | 33 ± 9 | 62 ± 9 |
Calculated monomeric MW from sequence | 15.7 | 32.2 | 47.9 |
Software employed | |||
Primary data reduction | SASFLOW | SASFLOW | SASFLOW |
Data processing | PRIMUS | PRIMUS | PRIMUS |
Rigid body modeling | CORAL | CORAL | SASREF |
Computation of model intensities | CRYSOL | CRYSOL | CRYSOL |
3D graphics representations | PYMOL | PYMOL | PYMOL |
