Fig. 3: Exchange behaviour in βPGM_WT.

Crystal structure of βPGM_WT (PDB 2WHE²⁹) showing residues of βPGM_WT undergoing conformational exchange on different timescales. Residues that populate two conformations in slow exchange are coloured in shades of blue according to chemical shift differences between conformer A and conformer B, with the intensity of colour and thickness of the backbone corresponding to larger values. Residues in conformer A and conformer B with missing backbone amide peaks in the ¹H¹⁵N-TROSY spectrum of βPGM_WT are coloured black, whereas missing backbone amide peaks in conformer B only are coloured purple. The amide ¹H¹⁵N coherences are likely broadened beyond detection owing to intermediate exchange on the millisecond timescale. The catalytic Mg²⁺ ion is indicated as a green sphere.