Fig. 7: Wnt inhibition partially rescues epidermal hyperplasia in the Grhl3−/− IFE. | Nature Communications

Fig. 7: Wnt inhibition partially rescues epidermal hyperplasia in the Grhl3−/− IFE.

From: Murine interfollicular epidermal differentiation is gradualistic with GRHL3 controlling progression from stem to transition cell states

Fig. 7

a GRHL3 ChIP-seq signal on the indicated Wnt signaling genes in the E16.5 epidermis. The red bars represent significant peaks. b Representative RNA-FISH images of Wnt4 (pink) and Lef1 (green) in Grhl3−/− P0 epidermis treated ex vivo with control (DMSO; left panel) or Wnt inhibitor (Wnt-C59; right panel). There is decreased Wnt4 and Lef1 expression in the epidermis treated with the Wnt inhibitor. Arrowheads point to signal. N = 3 biologically independent samples each. White broken line traces basal lamina. E epidermis, D dermis, HF hair follicle. c Representative sections of Grhl3−/− P0 epidermis treated ex vivo with control (DMSO; left panel) or Wnt inhibitor (Wnt-C59; right panel), representative image for N = 3 each. Krt14 staining, red; Krt10 staining, green; DAPI staining; blue; HF, hair follicle. There is decreased IFE thickening of the Wnt inhibitor treated epidermis. Scale bar = 10 µm. d Summary statistics for IFE thickness, mean and standard error for ex vivo Wnt inhibitor experiments. P-value was calculated with one-tailed student t-test. N = 3 biologically independent samples each.

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