Fig. 2: In silico validation of methanol-dependent strains. Methanol-dependent strains with gene deletions representing a single reaction knockout in FBA are shown.
From: Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
a The methanol-derived biomass fractions for predicted methanol-dependent strains growing on methanol with or without a sugar/sugar acid co-substrate, or a gluconeogenic co-substrate. The methanol-derived biomass fraction ranges from 0.0 (the biomass is methanol-independent) to 1.0 (the biomass fully depends on methanol). b The methanol-derived R5P fractions for predicted methanol-dependent strains. It ranges from 0.0 (completely independent) to 1.0 (completely dependent). c The ratio of the methanol-derived R5P fraction to the one of the biomass is used to compare the predicted methanol-dependent strains. The higher the ratio, the more promising the experimental implementation of the strain with an operational RuMP cycle (the top strains and conditions are highlighted by a black box). Source data are provided as a Source Data file.
