Fig. 5: Orai1 is ubiquitinated and degraded in a Crbn-dependent manner.

a–d The lysates of 293T cells transfected with the indicated plasmids were incubated with anti-FLAG antibody-conjugated agarose beads. Bead-bound proteins were detected with the indicated antibodies. Data are representative of three a, c, d or four b independent experiments. e BMDMs derived from the indicated mice were treated with MG132 (5 µM) for 6 h and then lysed. Proteins in the lysates were detected with the indicated antibodies (top) and the levels of Orai1 were quantified (bottom). n = 3 experiments, mean ± SEM. The numbers in the graph indicate p values. NS not significant (two-way ANOVA).