Fig. 6: Light-induced profiling of endogenous kinases and mitochondria-targeted labeling in live cells. | Nature Communications

Fig. 6: Light-induced profiling of endogenous kinases and mitochondria-targeted labeling in live cells.

From: Light-induced primary amines and o-nitrobenzyl alcohols cyclization as a versatile photoclick reaction for modular conjugation

Fig. 6

a Design of probe-1 targeting ATP binding site of kinase domain. Nitroso-probe-1 was photogenerated with temporal control. b Docking mode of STK24 kinase domain (PDB code:4QO9) with probe-1 (top), with photogenerated nitroso-probe-1 (bottom). c Venn diagrams showing the number of shared and unique kinases labeling with probe-1 from Jurkat cells and K562 cells, identified by LC–MS/MS after treatment of cells with probe-1. Captured protein kinases identified with ≥1 unique peptide, see Supplementary Information for details. d Schematic illustration of mitochondria-targeted temporal labeling via light-induced bioconjugation with fluorescent Rho-o-NBA probe in living cells. e Confocal images of MDA-MB-468 cells stained with Rho-o-NBA probe (red channel), Rhodamine 123 (green channel), and Hoechst 33342 (blue channel) in different conditions: colocalization of Rho-o-NBA probe and Rhodamine 123 without light activation (top), colocalization of Rho-o-NBA probe, and Mito-EGFP reporter under light activation (bottom), without light activation see Supplementary Fig. 20b.

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