Fig. 5: Fibrinogen covalent states are changed by mechanical shearing in vitro and in heart failure patients subjected to ECMO.
From: Fibrinogen function achieved through multiple covalent states
a Redox states of the five E region and eight D region fibrinogen disulfides of four different healthy donor plasmas sheared at rates of 2000 s−1 or 10000 s−1 for 5 min. The bars and errors are mean ± SD. Shearing at 10000 s−1 results in reduction of all 13 fibrinogen disulfides (p < 0.01 for the βC110-γC45 bond and p < 0.001 for the other disulfides). Parametric unpaired t-test was used to evaluate differences between groups. Source data are provided as a Source Data file. b Redox states of the five E region and eight D region fibrinogen disulfides in healthy donors (n = 10) versus patients with severe congestive cardiac failure subjected to ECMO (n = 8). The bars and errors are mean ± SD. Significant differences (p < 0.001) in the redox state of fibrinogen disulfides are indicated by stars. Parametric unpaired t-test was used to evaluate differences between groups. Source data are provided as a Source Data file.
