Fig. 7: αKG regulates BMP pathway.
From: Alpha-ketoglutarate ameliorates age-related osteoporosis via regulating histone methylations
a, b Gene set enrichment analysis (GSEA) showed increased expression of osteogenesis and BMP-regulated genes in αKG-treated MSCs. Cells isolated from aged mice were treated with 2 mM αKG for 48 h. c Heatmap of representative genes. d ChIP-qPCR assay revealed that αKG decreased the occupancy of H3K9me3 in the promoter regions of Bmp2 and Nanog (n = 3, by two-tailed Student’s t-test). e ChIP-qPCR showed that αKG decreased the occupancy of H3K27me3 in the promoter regions of Bmp2, Bmp4, and Nanog (n = 3, by two-tailed Student’s t-test). f Quantitative RT-PCR (n = 3, by one-way ANOVA with Tukey’s post hoc test). Histone demethylases inhibitor JIB 04 prohibited the effect of αKG on promoting Bmp2, Bmp4, and Nanog expression. g Western blot analyses of H3K9me3 and H3K27me3 in aged MSCs after supplementation of αKG and JIB 04. Data are shown as mean ± SEM.
