Fig. 2: Phenotypical analysis of M. xanthus single-, double-, and triple ask mutants under starvation compared to DK1622.

a FB formation of M. xanthus single-, double-, and triple ask mutants on CF starvation medium without additives, or in the presence of 20 nM ambruticin VS-3 or 100 mM glycerol. Pictures were taken after 48 h of incubation. Scale bars represent 0.5 mm. b Spores and pseudospores of M. xanthus single-, double-, and triple ask mutants produced during FB formation on CF starvation agar in presence or absence of glycerol or ambruticin VS-3. Pictures were taken after 48 h of incubation. Scale bars represent 1 µm. The experiments were performed in triplicates. c Effect of 60 nM ambruticin VS-3 or 300 mM glycerol on differentiation of single-, double-, and triple ask mutants into sonication resistant cells (dark gray bars) and on germination efficiency (light gray bars) after 96 h of development. The order of the single attempts is marked below each pair of columns (n.a. no additives; gly glycerol; VS3 ambruticin VS-3). N = 3 or 6 biologically independent samples. The data are presented as mean values ± SEM. Source data are provided as a Source Data file.