Fig. 2: Binding affinity and specificity of zan-DNP for viral neuraminidases.
From: A universal dual mechanism immunotherapy for the treatment of influenza virus infections
a Crystal structure of N1 neuraminidase of influenza A virus complexed with zanamivir (PDB code: 3TI5). Note that the C-7 hydroxyl of zanamivir is exposed to solvent and available for linkage to DNP. b Structure of zanamivir-rhodamine conjugate (zan-rhodamine). c–f Binding of zan-rhodamine (direct binding curve, left panel), zanamivir (competitive binding curve, central panel), and zan-DNP (competitive binding curve, right panel) to neuraminidase expressed on MDCK cells infected with influenza virus A/Wisconsin/629-D00015/2009 (H1N1)pdm09 (c), A/Netherlands/22/2003 (H3N2) (d), B/Florida/4/2006 (Yamagata Lineage) (e) or B/Brisbane/60/2008 (Victoria Lineage) (f). All the virus-infected cells were used 24 h post-infection for binding studies. Data are presented as mean values ± SD (n = 3).
