Fig. 4: Fluctuations and spontaneous transitions at the low-density limit.
From: From deterministic to fuzzy decision-making in artificial cells
a Fano factor (variance over mean) with Poissonian process (dashed line) for the GRNs at various temperatures (upper row). Amplitude of temporal autocorrelation function (ACF) of fluctuating production rates (as shown in Figs. 2c, 3a) in individual compartments at τ = 30 s. Error bars were bootstrapped and show mean and SD of compartments (see Supplementary Fig. 12 for the bistable and monostable GRN and Supplementary Fig. 14 for the wild-type monostable GRN). b Scheme of noise, active promoter transitions, and memory in the bistable GRN. Short-term fluctuations in gene expression (circle within a well) originate from local noise. Long-term fluctuations can originate from spontaneous promoter transitions with the deactivation of CIts. c Number of proteins produced in 15 s for long-term experiment of the bistable GRN at 37 °C. d The ACF of the trace in panel c. Source data are provided as a Source data file.
