Fig. 7: IFNAR deletion abrogates NET formation prompted by GM-CSF blockade during infection. | Nature Communications

Fig. 7: IFNAR deletion abrogates NET formation prompted by GM-CSF blockade during infection.

From: Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis

Fig. 7

WT (circles) and Ifnar−/− (triangles) mice were infected and treated with Ctrl Ab (closed symbols) or αGM-CSF (open symbols) as in Fig. 1a. a Representative images of lung sections stained with citrullinated histone H3 (citH3; red), MPO (green), and DAPI (blue). NETs are visualized by colocalization of citH3 and DAPI staining (merged images; n = 3 mice/group). Scale bars, 50 µm. Data representative of two biological experiments. b Percentage of NET area in the lung normalized to MPO positive signal. **P = 0.0022. Data pooled from two biological experiments. Represented is the mean ± SD; each dot represents an individual mouse: n = 6 mice/group. Source data are provided as a Source Data file. Statistical analysis was performed using the two-tailed Mann–Whitney test.

Back to article page